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goat anti rat af647  (Jackson Immuno)
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(A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, <t>cetuximab-AF647.</t> (C,D) Displacement titration of <t>cetuximab</t> (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.
Cetuximab Af647, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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af647 conjugated streptavidin  (Jackson Immuno)
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(A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, <t>cetuximab-AF647.</t> (C,D) Displacement titration of <t>cetuximab</t> (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.
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(A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, <t>cetuximab-AF647.</t> (C,D) Displacement titration of <t>cetuximab</t> (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.
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sqstm1 p62 af647 novus biologicals nbp1 42821af647 facs  (Novus Biologicals)
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(A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, <t>cetuximab-AF647.</t> (C,D) Displacement titration of <t>cetuximab</t> (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.
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(A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, <t>cetuximab-AF647.</t> (C,D) Displacement titration of <t>cetuximab</t> (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.
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(A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, cetuximab-AF647. (C,D) Displacement titration of cetuximab (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.

Journal: bioRxiv

Article Title: High-throughput affinity profiling of antibodies against soluble and native membrane targets by digital flow cytometry

doi: 10.64898/2026.10.15.759441

Figure Lengend Snippet: (A) Schematic of the displacement titration assay. An unlabeled antibody or antibody-drug conjugate (ADC) is titrated against a fixed concentration of fluorescent reference antibody (“tracer”) in equilibrium with EVs presenting the target membrane receptor. Redistribution of the tracer is used to determine the K D of the unlabeled competitor. (B) Equilibrium titration (without competition; assay shown in ) to determine the K D of the tracer, cetuximab-AF647. (C,D) Displacement titration of cetuximab (C) and cetuximab-MMAE (D) using cetuximab-AF647 as tracer. (E) Three-point competition assay for relative affinity measurements of unlabeled competitors. (F) Relative affinity measurements of cetuximab and cetuximab-MMAE across multiple competitor-to-tracer ratios.

Article Snippet: Cetuximab-AF647 (clone C225; Novus Biologicals, CO) was titrated over ten concentrations: 4.15 fM, 12.45 fM, 41.5 fM, 124.5 fM, 208 fM, 415 fM, 0.83 pM, 2.08 pM, 4.15 pM, and 8.3 pM.

Techniques: Titration, Concentration Assay, Membrane, Competitive Binding Assay, Relative Affinity